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rabbit polyclonal antihuman ccl19  (Novus Biologicals)


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    Novus Biologicals rabbit polyclonal antihuman ccl19
    Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and <t>CCL19</t> protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.
    Rabbit Polyclonal Antihuman Ccl19, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antihuman ccl19/product/Novus Biologicals
    Average 91 stars, based on 2 article reviews
    rabbit polyclonal antihuman ccl19 - by Bioz Stars, 2026-02
    91/100 stars

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    1) Product Images from "The Gene Signature of Activated M-CSF-Primed Human Monocyte-Derived Macrophages Is IL-10-Dependent"

    Article Title: The Gene Signature of Activated M-CSF-Primed Human Monocyte-Derived Macrophages Is IL-10-Dependent

    Journal: Journal of Innate Immunity

    doi: 10.1159/000519305

    Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and CCL19 protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.
    Figure Legend Snippet: Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and CCL19 protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.

    Techniques Used: Fluorescence, Microarray, Gene Expression, Comparison, Expressing, Quantitative RT-PCR, Western Blot, Derivative Assay



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    Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and <t>CCL19</t> protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.
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    Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and <t>CCL19</t> protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.
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    Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and <t>CCL19</t> protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.
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    Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and CCL19 protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.

    Journal: Journal of Innate Immunity

    Article Title: The Gene Signature of Activated M-CSF-Primed Human Monocyte-Derived Macrophages Is IL-10-Dependent

    doi: 10.1159/000519305

    Figure Lengend Snippet: Identification of genes differentially regulated by LPS in M-MØ and GM-MØ. a Experimental design. b Normalized fluorescence intensity of the indicated cytokine mRNA in untreated and LPS-treated M-MØ and GM-MØ. c Scatter plot of microarray results, showing the LPS-induced gene expression changes in M-MØ (log2 M-MØ + LPS/M-MØ, adj p < 0.05, x-axis) plotted against the difference in the LPS-induced gene expression changes in M-MØ and GM-MØ (log2FC [M-MØ + LPS/M-MØ] − log2FC [GM-MØ + LPS/GM-MØ]) (y-axis). The relative position of some informative genes is indicated. d GSEA on the ranked list of genes obtained from the comparison of the transcriptome of M-MØ + LPS versus GM-MØ + LPS using Gene set 1 genes. The identity of the genes within the leading edge is shown. e Identification of the gene sets including genes with the highest differential LPS responsiveness in M-MØ (Gene set 1) and GM-MØ (Gene set 2). The number of genes in each gene set and associated gene ontology terms (Enrichr) are indicated. f Expression of selected members of Gene set 1 in untreated and LPS-treated M-MØ and GM-MØ (left panel), untreated and HMGB1-treated M-MØ and GM-MØ (middle panel), and untreated and PAM3CSK4-treated M-MØ (right panel), as determined by qRT-PCR on 4 independent samples. Results are indicated as the mRNA levels of each gene in activated cells relative to the level of the same mRNA in untreated cells (n = 3–4; *, p < 0.05; **, p < 0.005; ***, p < 0.0005). g SOCS2 protein levels in GM-MØ and M-MØ stimulated with LPS for the indicated times, as determined by Western blot. Shown is 1 representative experiment (n = 2). hCCL19 mRNA and CCL19 protein levels in untreated (−) and LPS-treated M-MØ and GM-MØ. Shown are the means and SD of 5 independent experiments (n = 5; *, p < 0.05). i Expression of the indicated Gene set 1 genes in M-MØ stimulated with LPS (4 h) in the presence of U0126, BIRB0796, or BIRB0796 and U0126. Results indicate the expression of each gene relative to its expression in LPS-stimulated M-MØ. Shown are the means and SD of 3–4 independent experiments (n = 3–4; *, p < 0.05; **, p < 0.01; ***, p < 0.005). LPS, lipopolysaccharide; GSEA, gene set enrichment analysis; M-MØ, monocyte-derived human macrophages.

    Article Snippet: After deparaffinization, rehydration, and antigen-retrieval using the automated PT Link system (Dako, Agilent Technologies), tissue microarray slides were incubated with a mixture of a mouse monoclonal antihuman CD163 (NB110-59935; Novus Biologicals) and a rabbit polyclonal antihuman CCL19 (NBP2-56275; Novus Biologicals) overnight at 4°C.

    Techniques: Fluorescence, Microarray, Gene Expression, Comparison, Expressing, Quantitative RT-PCR, Western Blot, Derivative Assay